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Description
Research Area
Signal Transduction
Images & Validation
−
| Tested Applications | ELISA, WB |
|---|---|
| Dilution Range | ELISA: 1:2,000 - 1:10,000, WB: 1:500-1:2000 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Anti-RON Antibody was produced in rabbits by repeated immunizations with a recombinant protein produced in Sf9 cells corresponding to the full length human RON protein. |
| Target | MST1R |
| Purity | Anti-RON antibody is directed against human RON. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-RON antibody was purified from rabbit serum by Protein A chromatography and cross adsorption of the GST tag. Cross reactivity with RON from other species has not been determined. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1.0 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-RON antibody, anti-MSP receptor, CDw136, CD136, Macrophage-stimulating protein receptor, p185-Ron, PTK8, RON, Protein-tyrosine kinase 8, c-met-related tyrosine kinase
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Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_002438.2 |
|---|
Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)












