ROCK2 Antibody

SKU: orb1239115

Description

ROCK2 Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Porcine
• Application Notes:
  ROCK2 antibody can be used for detection of ROCK2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: ROCK2 antibody was raised against a 15 amino acid synthetic peptide near the center of human ROCK2.The immunogen is located within amino acids 920 - 970 of ROCK2.
• Target: ROCK2
• Molecular Weight: Predicted: 153 kDa Observed: 160 kDa
• Purification: ROCK2 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: ROCK2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ROCK2 Antibody: ROCK-II, KIAA0619, Rho-associated protein kinase 2, Rho kinase 2, ROCK-II

Western blot analysis of ROCK2 in mouse brain tissue lysate with ROCK2 antibody at (A) 1 and (B) 2 µg/mL.

Immunohistochemistry of ROCK2 in mouse brain tissue with ROCK2 antibody at 5 µg/mL.

Immunofluorescence of ROCK2 in mouse brain tissue with ROCK2 antibody at 20 µg/mL.

Quick Database Links

Gene Symbol

ROCK2

UniProt

O75116

RefSeq (Protein):NP_004841