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| Catalog Number | orb1239115 |
|---|---|
| Category | Antibodies |
| Description | ROCK2 Antibody |
| Target | ROCK2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Bovine, Porcine |
| Form/Appearance | Liquid |
| Concentration | 1 mg/mL |
| Buffer/Preservatives | ROCK2 Antibody is supplied in PBS containing 0.02% sodium azide. |
| Purification | ROCK2 Antibody is affinity chromatography purified via peptide column. |
| Immunogen | ROCK2 antibody was raised against a 15 amino acid synthetic peptide near the center of human ROCK2.The immunogen is located within amino acids 920 - 970 of ROCK2. |
| UniProt ID | O75116 |
| MW | Predicted: 153 kDa Observed: 160 kDa |
| Tested applications | ELISA, IF, IHC-P, WB |
| Application notes | ROCK2 antibody can be used for detection of ROCK2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | ROCK2 Antibody: ROCK-II, KIAA0619, Rho-associated Read more... |
| Research Area | Cell Biology |
| Note | For research use only |
| NCBI | NP_004841 |

Western blot analysis of ROCK2 in mouse brain tissue lysate with ROCK2 antibody at (A) 1 and (B) 2 µg/mL.

Immunohistochemistry of ROCK2 in mouse brain tissue with ROCK2 antibody at 5 µg/mL.

Immunofluorescence of ROCK2 in mouse brain tissue with ROCK2 antibody at 20 µg/mL.
IF, IHC-Fr, IHC-P, WB | |
Rabbit, Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
Human | |
0.16-10 ng/mL | |
0.059 ng/mL |
Human | |
0.32-20 ng/mL | |
0.113 ng/mL |
Human | |
0.32-20 ng/mL | |
0.13 ng/mL |
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