RNF43 Antibody (C-term)

• Catalog Number: orb1936850
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB32930
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:2000, WB: 1:1000, IHC-P: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 85722 Da
• Target: This RNF43 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 543-572 amino acids from the C-terminal region of human RNF43.
• UniProt ID: Q68DV7
• NCBI: NP_060233.3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: E3 ubiquitin-protein ligase RNF43, 632-, RING fing
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-RNF43 Antibody (C-term) at 1:2000 dilution + A549 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 86 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

RNF43 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of RNF43 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

RNF43 Antibody (C-term) western blot analysis in CEM cell line lysates (35 ug/lane). This demonstrates the RNF43 antibody detected the RNF43 protein (arrow).