RNF31 Antibody (C-term)

• Catalog Number: orb1932443
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB39346
• Tested applications: WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:2000, WB: 1:2000, WB: 1:2000, WB: 1:1000
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 119652 Da
• Target: This RNF31 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 955-983 amino acids from the C-terminal region of human RNF31.
• UniProt ID: Q96EP0
• NCBI: NP_060469.4
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: E3 ubiquitin-protein ligase RNF31, 632-, HOIL-1-in
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

RNF31 Antibody (C-term) western blot analysis in CEM cell line lysates (35 ug/lane). This demonstrates the RNF31 antibody detected the RNF31 protein (arrow).

All lanes: Anti-RNF31 Antibody (C-term) at 1:2000 dilution. Lane 1: 293T/17 whole cell lysate. Lane 2: CEM whole cell lysate. Lane 3: Hela whole cell lysate. Lane 4: HepG2 whole cell lysate. Lane 5: Jurkat whole cell lysate. Lane 6: MCF-7 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 120 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-RNF31 Antibody (C-term) at 1:2000 dilution. Lane 1: 293T/17 whole cell lysate. Lane 2: CCRF-CEM whole cell lysate. Lane 3: Hela whole cell lysate. Lane 4: Jurkat whole cell lysate. Lane 5: MDA-MB-453 whole cell lysate. Lane 6: MCF-7 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 58 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-RNF31 Antibody (C-term) at 1:2000 dilution. Lane 1: 293T/17 whole cell lysate. Lane 2: CCRF-CEM whole cell lysate. Lane 3: Hela whole cell lysate. Lane 4: Jurkat whole cell lysate. Lane 5: MDA-MB-453 whole cell lysate. Lane 6: MCF-7 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 58 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.