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Catalog Number | orb1260235 |
---|---|
Category | Antibodies |
Description | RNF17 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 900-1200 of human RNF17 (NP_112567.2). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 185kDa |
Target | RNF17 |
UniProt ID | Q9BXT8 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | Mmip-2, SPATA23, TDRD4, RING finger protein 17, sp Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of A-549 cells, using RNF17 antibody (orb1260235) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
ELISA, IF, IHC-Fr, IHC-P | |
Bovine, Canine, Equine, Human, Mouse, Sheep | |
Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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