RNF149 Antibody

• Catalog Number: orb1259417
• Category: Antibodies
• Description: RNF149 Antibody
• Target: RNF149
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 221-400 of human RNF149 (NP_775918.2).
• UniProt ID: Q8NC42
• MW: Observed: 43kDa
• Tested applications: IF, IHC, WB
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: E3 ubiquitin-protein ligase RNF149, 632-, DNA poly
• Research Area: Cell Biology
• Note: For research use only

Western blot analysis of extracts of various cell lines, using RNF149 antibody (orb1259417) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunofluorescence analysis of C6 cells using RNF149 Polyclonal Antibody (orb1259417) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.