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RNF133 Antibody (Center)
Catalog Number: orb1933889
| Catalog Number | orb1933889 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB37620 |
| Tested applications | WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, WB: 1:1000 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 42294 Da |
| Target | This RNF133 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 245-271 amino acids from the Central region of human RNF133. |
| UniProt ID | Q8WVZ7 |
| NCBI | NP_631914.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | E3 ubiquitin-protein ligase RNF133, 632-, RING fin Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
RNF133 Antibody (Center) western blot analysis in NCI-H292 cell line lysates (35 ug/lane). This demonstrates the RNF133 antibody detected the RNF133 protein (arrow).
Anti-RNF133 Antibody (Center) at 1:1000 dilution + K562 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 42 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
