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Catalog Number | orb1259872 |
---|---|
Category | Antibodies |
Description | RNASE12 Antibody |
Target | RNASE12 |
Clonality | Polyclonal |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Mouse, Rat |
Form/Appearance | Liquid |
Concentration | batch dependent |
Buffer/Preservatives | Liquid |
Purification | Affinity purification |
Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RNASE12 (NP_001019993.1). |
UniProt ID | Q5GAN4 |
MW | Observed: 17kDa |
Tested applications | WB |
Application notes | WB: 1:500 - 1:2000 |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | HEL-S-85p, RAI1, probable inactive ribonuclease-li Read more... |
Note | For research use only |
Western blot analysis of extracts of various cell lines, using RNASE12 antibody (orb1259872) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.