RN185 Antibody (Center)

SKU: orb1938678

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cell Biology, Protein Biochemistry

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: WB - 1:1000, FC - 1:10-50
• Reactivity: Hamster, Human, Mouse
• Predicted Reactivity: Rat, Zebrafish

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD40371
• Immunogen: This RN185 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 88-117 amino acids from the Central region of human RN185. Antigen Region: 88-117 aa.
• Target: RNF185 {ECO:0000303|Ref.1, ECO:0000312|HGNC:HGNC:26783}
• Molecular Weight: 20459 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

E3 ubiquitin-protein ligase RNF185, 632-, RING finger protein 185, RNF185

RN185 Antibody (Center) western blot analysis in CHO cell line lysates (35 ug/lane). This demonstrates the RN185 antibody detected the RN185 protein (arrow).

RN185 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

RN185 Antibody (Center) western blot analysis in mouse kidney tissue and K562 cell line lysates (35 ug/lane). This demonstrates the RN185 antibody detected the RN185 protein (arrow).

Quick Database Links

Gene Symbol

RNF185 {ECO:0000303|Ref.1, ECO:0000312|HGNC:HGNC:26783}

UniProt

Q96GF1

RefSeq (Protein):NP_689480.2, NP_001129297.1