RI-1

SKU: orb1305819

Description

RI-1

Research Area

Cell Biology, Molecular Biology

Key Properties

• CAS Number: 415713-60-9
• MW: 361.61
• Purity: 99.30%
• Formula: C₁₄H₁₁Cl₃N₂O₃
• SMILES: ClC1=C(N2CCOCC2)C(=O)N(C1=O)c1ccc(Cl)c(Cl)c1
• Target: DNA/RNA Synthesis
• Solubility: DMSO:36.2 mg/mL (100.11 mM);10% DMSO+90% Corn Oil:2 mg/mL (5.53 mM)

Bioactivity

• Target IC50:
  RAD51 < 0 μM
• In Vivo:
  PluriSIns 1 showed Single-agent toxicity (LD50: 20-40 μM) in three cancer cell lines: HeLa cells, MCF-7 and U2OS. Through Direct and specific disruption of HsRAD51 and Inhibition of RAD51, RI-1 inhibite the ability of RAD51 to form filaments on ssDNA, resulting in increased cellular susceptibility to DNA damage.RI-1 caused a decrease in γ-H2AX foci in G2-phase cells and an increase in the level of unrepaired DSBs 6 h after radiation.
• Cell Research:
  Cytotoxicity is determined by loss of colony-forming ability. Experiments are performed in triplicate. Crystal violet stained colonies are imaged with a CCD camera and counted using NIH Image software. Error bars denote s andard error.(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Disclaimer: For research use only

Alternative Names

RAD51 inhibitor 1, RAD51, recombination, RI 1, RI1, RI-1, RNA Synthesis, RNASynthesis, DNA Synthesis, DNASynthesis, inhibit, Inhibitor, homologous

Compound Identifiers

PubChem CID

1074953