RI-1

SKU: orb1305819

Description

RI-1 is a small molecule inhibitor of the RAD51 recombinase, exhibiting inhibitory activity with an IC50 in the 5-30 µM range. It is utilized in research to study DNA repair mechanisms, particularly homologous recombination, and has been applied in both cellular and biochemical assays to investigate cancer biology and potential therapeutic strategies.

Research Area

Cell Biology, Molecular Biology

Key Properties

• CAS Number: 415713-60-9
• MW: 361.61
• Purity: 99.92% (May vary between batches)
• Formula: C₁₄H₁₁Cl₃N₂O₃
• SMILES: ClC1=C(N2CCOCC2)C(=O)N(C1=O)c1ccc(Cl)c(Cl)c1
• Target: DNA/RNA Synthesis
• Solubility: DMSO:36.2 mg/mL (100.11 mM);10% DMSO+90% Corn Oil:2 mg/mL (5.53 mM)

Bioactivity

• Target IC50:
  RAD51:<30 μM
• In Vivo:
  PluriSIns 1 showed single-agent toxicity (LD50: 20-40 μM) in three cancer cell lines: HeLa cells, MCF-7 and U2OS. Through direct and specific disruption of HsRAD51 and inhibition of RAD51, RI-1 inhibited the ability of RAD51 to form filaments on ssDNA, resulting in increased cellular susceptibility to DNA damage.RI-1 caused a decrease in γ-H2AX foci in G2-phase cells and an increase in the level of unrepaired DSBs 6 h after radiation.
• Cell Research:
  Cytotoxicity is determined by loss of colony-forming ability. Experiments are performed in triplicate. Crystal violet stained colonies are imaged with a CCD camera and counted using NIH Image software. Error bars denote standard error.(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

RAD51 inhibitor 1, RAD51, recombination, RI 1, RI1, RI-1, RNA Synthesis, RNASynthesis, DNA Synthesis, DNASynthesis, inhibit, Inhibitor, homologous

Compound Identifiers

PubChem CID

1074953