Rhodopsin Antibody (APC)

SKU: orb147737

Description

Mouse monoclonal to Rhodopsin (APC). Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photo reactive chromophore, in a central pocket. In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption. In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsins activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin. Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder..

Research Area

Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC, IP, WB
• Dilution Range: WB (1:1000), IHC (1000)
• Reactivity: Amphibian, Aves, Bovine, Fish, Human, Mouse
• Application Notes:
  1 µg/ml was sufficient for detection of rhodopsin in 10 µg of rat eye lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 4D2
• Immunogen: Bovine Rhodopsin
• Target: Rhodopsin
• Molecular Weight: 40kDa
• Purification: Protein G Purified
• Conjugation: APC

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 95.46mM Phosphate, 2.48mM MES and 2mM EDTA
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

OPN2, opsd, opsin 2, opsin 2 rod pigment, opsin2, RHO, RP4, MGC138309, Retinitis Pigmentosa 4

Quick Database Links

Gene Symbol

Rhodopsin

UniProt

P02699

NCBI Gene ID

509933

RefSeq (Protein):NP_001014890.1