Rho H rabbit pAb Antibody

SKU: orb768993

Description

Rabbit polyclonal antibody to Rho H

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution Range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human RhoH. AA range:141-190
• Molecular Weight: 21
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-RHOH antibody, anti-ARHH antibody, anti-TTF antibody, anti-Rho-related GTP-binding protein RhoH antibody, anti-GTP-binding protein TTF antibody, anti-Translocation three four protein antibody

Immunofluorescence analysis of A549 cells, using RhoH Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HT-29 cells, using RhoH Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of the lysates from RAW264.7cells using RhoH antibody.

Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200.