RGS1 Antibody

• Catalog Number: orb1538096
• Category: Antibodies
• Description: RGS1 Antibody
• Target: RGS1
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Bovine, Equine, Human, Mammal, Monkey
• Concentration: 0.5 mg/ml
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: Peptide with sequence C-NLLNDLQANSLK, from the C-Terminus of protein sequence according to NP_002913.3.
• Tested applications: ELISA, IHC, IHC-P, WB
• Dilution range: IHC, IHC-P (2.5 µg/ml), Peptide-ELISA (1:32000), WB (0.5 - 2 µg/ml)
• Application notes: Further information: Immunohistochemistry: LS-B3594 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3594 was determined to be 2.5 ug/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: RGS1, 1R20, B-cell activation protein BL34, BL34,
• Note: For research use only

RGS1 / 1R20 antibody staining (1 µg/ml) of HepG2 lysate (RIPA buffer, 30 µg total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.