RFX5 Antibody

SKU: orb1248487

Description

RFX5 Antibody

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Porcine, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 70kDa band observed in lysates of cell line Daudi and in NIH3T3 nuclear lysates (calculated MW of 65.3kDa according to NP_000440.1). Recommended concentration: 1-3µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-TGDKSSEPSTLSNE
• Target: RFX5
• Molecular Weight: Approx 70kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

RFX5, regulatory factor X, 5 (influences HLA class II expression), OTTHUMP00000196318, regulatory factor X, 5

orb1248487 (2 ug/ml) staining of Daudi lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248487 (2 ug/ml) staining of NIH3T3 nuclear lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

RFX5

UniProt

P48382

RefSeq (Protein):NP_000440.1