RFX5 Antibody

• Catalog Number: orb1248487
• Category: Antibodies
• Description: RFX5 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Predicted Reactivity: Bovine, Canine, Porcine, Rat
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: C-TGDKSSEPSTLSNE
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 70kDa band observed in lysates of cell line Daudi and in NIH3T3 nuclear lysates (calculated MW of 65.3kDa according to NP_000440.1). Recommended concentration: 1-3ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 70kDa
• Target: RFX5
• UniProt ID: P48382
• NCBI: NP_000440.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: RFX5, regulatory factor X, 5 (influences HLA class
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 70kDa band observed in lysates of cell line Daudi and in NIH3T3 nuclear lysates (calculated MW of 65.3kDa according to NP_000440.1). Recommended concentration: 1-3ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248487 (2 ug/ml) staining of Daudi lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248487 (2 ug/ml) staining of NIH3T3 nuclear lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.