RFX1 (vPairTM) Antibodies

SKU: orb131895

Description

Rabbit polyclonal to RFX1. RFX1 antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Biorbyt to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody pairs-based assays. The use of a pair of fully characterized vPair antibodies in the same assay can validate signal specificity since vPair antibodies recognize two independent epitopes of the same protein. Different sets of vPair antibodies are developed at Biorbyt to work with specific applications, including antibody pairs arrays, Western blot, IP-Western, ChIP, IHC, and FACS. Regulatory factor X 1 (RFX1) is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. RFX1 is structurally related to regulatory factors X2, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with RFX family members X2, X3, and X5, but not with X4. RFX1 binds to the X-boxes of MHC class II genes and is essential for their expression. Also, it can bind to an inverted repeat required for expression of hepatitis B virus genes.

Research Area

Epigenetics

Images & Validation

• Tested Applications: ChIP, IP, WB
• Dilution Range: WB:1:1000-1:3000
• Reactivity: Human
• Application Notes:
  WB: The apparent protein size on WB may be different from the calculated M.W. due to modifications.; ; Experiment Notes: Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the N-terminal or the C-terminal region of human RFX1.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: N-terminal or the C-terminal region of human RFX1
• Molecular Weight: 105
• Purity: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01% NaN3
• Concentration: 100 μg/ml BSA
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti MHC class II regulatory factor RFX1 antibody pairs, anti Enhancer factor C antibody pairs, anti EF-C antibody pairs, anti Regulatory factor X 1 antibody pairs, anti RFX antibody pairs, anti Transcription factor RFX1 antibody pairs

Quick Database Links

UniProt

P22670