RFC4 Antibody

• Catalog Number: orb1257462
• Category: Antibodies
• Description: RFC4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 204-363 of human RFC4 (NP_002907.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 40kDa
• Target: RFC4
• UniProt ID: P35249
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: RFC4, A1, MGC27291, RFC37
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using RFC4 antibody (orb1257462) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry of paraffin-embedded rat testis using RFC4 Antibody (orb1257462) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat testis using RFC4 Antibody (orb1257462) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human breast cancer using RFC4 Antibody (orb1257462) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using RFC4 antibody (orb1257462). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using RFC4 antibody (orb1257462). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of K562 cells using 1 ug RFC4 antibody (orb1257462). Western blot was performed from the immunoprecipitate using RFC4 antibody (orb1257462) at a dilution of 1:1000.