RFC2 Antibody (N-term)

• Catalog Number: orb1931449
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB17303
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Rat
• Reactivity: Human, Rat
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 39157 Da
• Target: This RFC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-46 amino acids from the N-terminal region of human RFC2.
• UniProt ID: P35250
• NCBI: NP_002905.2, NP_001265722.1, NP_001265721.1, NP_852136.1, NP_001265720.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Replication factor C subunit 2, Activator 1 40 kDa
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

RFC2 Antibody (N-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Rat lung, taken with 40x objective. Immunohistochemistry with RFC2 Antibody (N-term), 1:200 dilution, counter stained with Hematoxylin. Positive cells identified with arrows. Av-Alveoli, Br-Bronchus, Pa-Pulmonary Artery.

All lanes: Anti-RFC2 Antibody (N-term) at 1:1000 dilution. Lane 1: K562 whole cell lysate. Lane 2: Hela whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 39 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.