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RF1 Antibody
Description
Images & Validation
−| Tested Applications | FC, WB |
|---|---|
| Dilution range | WB: 1/500 - 1/2000; FCM: 1/200 - 1/400; ELISA: 1/10000 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | 4F9H12 |
| Immunogen | Purified recombinant fragment of human RF1 (AA: 288-437) expressed in E. Coli. |
| Target | RF1 |
| Molecular Weight | 49kDa |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody in PBS with 0.05% sodium azide |
| Concentration | Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−EBP50/NHERF-1/SLC9A3R1 Antibody [orb546330]
ELISA, IHC, WB
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
100 μg

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Flow cytometric analysis of HepG2 cells using RF1 antibody

Western blot analysis of human recombinant protein using RF1 antibody

Western blot analysis of HEK293 using RF1 antibody

Western blot analysis of MCF-7 (Lane1), T47D (Lane2), MOLT4 (Lane3), and Raji (Lane4) cell lysate using RF1 antibody

ELISA Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Flow cytometric analysis of Hela cells using RF1 antibody
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Protocol Information
RF1 Antibody (orb333891)
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