Retinoblastoma Antibody / Rb1

• Catalog Number: orb639503
• Category: Antibodies
• Description: Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It reacts with the hyper-phosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: RB1/1754
• Tested applications: IHC-P
• Reactivity: Human, Mouse
• Isotype: Mouse IgG1, kappa
• Immunogen: A human recombinant protein was used as the immunogen for this Rb antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Rb antibody is available for research use only.
• UniProt ID: P06400
• Storage: The Rb antibody (with azide) can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Titering of the Rb antibody may be required for optimal performance.
• Expiration Date: 12 months from date of receipt.

IHC testing of human colon with Rb antibody (clone RB1/1754). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of human colon with Rb antibody (clone RB1/1754). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.