alfa Human Monoclonal Antibody

SKU: orb2978107

Description

Simlukafusp alfais a research-grade recombinant antibody targeting Surface-expressed protease. Produced in mammalian cells with native-like glycosylation. Highlights Research Grade: For PK/PD studies, assay development, and ADA research. Native Glycosylation: Mammalian expression ensures native-like patterns.

Images & Validation

• Tested Applications: ELISA, FA, FACS, In vivo
• Reactivity: Human

Key Properties

• Antibody Type: Biosimilar Antibody
• Host: Human
• Clonality: Monoclonal
• Isotype: IgG1-kappa
• Source/Expression System: Mammalian Cells
• Purity: >95% purity as determined by SDS-PAGE.
• Purification: Protein A/G purified from cell culture supernatant.
• Endotoxin: Please contact with the lab for this information.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M PBS, pH 7.4.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Surface-expressed protease, Gelatine degradation protease FAP, Integral membrane serine protease, Dipeptidyl peptidase FAP, Seprase, FAP, Post-proline cleaving enzyme, APCE, SIMP, FAPalpha, 170 kDa melanoma membrane-bound gelatinase, Fibroblast activation protein alpha, Serine integral membrane protease, Prolyl endopeptidase FAP

Detects FAP/FAPalpha in indirect ELISAs.

Flow-cytometry using anti-human FAP antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human FAP monoclonal antibody (Catalog: orb2978107) at a concentration of 5 µg/mL for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using FITC anti-human FAP&IL2V antibody. U87 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human FAP&IL2V monoclonal antibody (Catalog: orb2978107, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE for Research Grade Simlukafusp alfa.

Western blot analysis was performed using anti-FAP monoclonal antibody at 1 µg/mL on various samples. Lane 1: recombinant human FAP (Catalog No: orb2966744), 50 ng, Lane 2: recombinant human FAP (Catalog No: orb2966744), 30 ng, Lane 3: recombinant human FAP (Catalog No: orb2966744), 20 ng.

Quick Database Links

UniProt

Q12884