CD66e/CEA/CEACAM5 Human Monoclonal Antibody

SKU: orb2977373

Description

Anti-Human CD66e/CEA/CEACAM5 (BUIO323) is a research-grade recombinant antibody targeting CD66. Produced in mammalian cells with native-like glycosylation. Highlights Research Grade: For PK/PD studies, assay development, and ADA research. Native Glycosylation: Mammalian expression ensures native-like patterns.

Research Area

Cancer Biology; Cell Biology

Images & Validation

• Tested Applications: ELISA, FA, FACS, In vivo
• Reactivity: Human

Key Properties

• Antibody Type: Biosimilar Antibody
• Host: Human
• Clonality: Monoclonal
• Isotype: IgG1-kappa
• Clone No.: BUIO323
• Source/Expression System: Mammalian Cells
• Purity: >95% purity as determined by SDS-PAGE.
• Purification: Protein A/G purified from cell culture supernatant.
• Endotoxin: Please contact with the lab for this information.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M PBS, pH 7.4.
• Concentration: 3.13 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Meconium antigen 100, CEACAM5, CEA, CD66e, Carcinoembryonic antigen-related cell adhesion molecule 5, Carcinoembryonic antigen

Detects recombinant human CD66e/CEA/CEACAM5 (Cat No.: orb2969466) in indirect ELISA.

Flow-cytometry using FITC anti-human CD66e antibody. HPAC cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD66e monoclonal antibody (Catalog orb2977373, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using PE anti-human CD66e antibody. HPAC cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD66e monoclonal antibody (Catalog orb2977373, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE for Research Grade Anti-Human CD66e/CEA/CEACAM5.

Western blot analysis was performed using anti-CD66e/CEA/CEACAM5 monoclonal antibody at 1 µg/mL on various samples. Lane 1: recombinant human CD66e/CEA/CEACAM5 (Catalog No: orb2969466), Lane 2: negative control.

Quick Database Links

UniProt

P06731