CD50/ICAM3 Human Monoclonal Antibody

SKU: orb2977994

Description

Anti-Human CD50/ICAM3 (BKEO5) is a research-grade recombinant antibody targeting CD50. Produced in mammalian cells with native-like glycosylation. Highlights Research Grade: For PK/PD studies, assay development, and ADA research. Native Glycosylation: Mammalian expression ensures native-like patterns.

Research Area

Neuroscience; Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, FA, FACS, In vivo
• Reactivity: Human

Key Properties

• Antibody Type: Biosimilar Antibody
• Host: Human Humanized
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: BKEO5
• Purity: >95% purity as determined by SDS-PAGE.
• Purification: Protein A/G purified from cell culture supernatant.
• Endotoxin: Please contact the lab for this information.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M PBS, pH 7.4.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD50, CDw50, Intercellular adhesion molecule 3, ICAM-R, ICAM3, ICAM-3

Flow-cytometry using anti-human CD50 antibody. U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD50 monoclonal antibody (Catalog: orb2977994, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using APC anti-human CD50 antibody. RPMI-8226 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD50 monoclonal antibody (Catalog: orb2977994, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using FITC anti-human CD50 antibody. RPMI-8226 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD50 monoclonal antibody (Catalog: orb2977994, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using PE anti-human CD50 antibody. RPMI-8226 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD50 monoclonal antibody (Catalog: orb2977994, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE for Research Grade Anti-Human CD50/ICAM3.

Quick Database Links

UniProt

P32942