RELA/NFKB p65 Antibody

SKU: orb1538812

Description

RELA/NFKB p65 Antibody

Images & Validation

• Tested Applications: ChIP, ELISA, Enzyme Assay, IHC, IHC-P, WB
• Dilution Range: ChrIP, ELISA (1:5000), GS, IHC, IHC-P (1:500), WB (1:2000 - 1:5000)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 1:500.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: NFkB p65 (Rel A) peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
• Target: RELA / NFKB p65
• Purification: Delipidated and defibrinated
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01% Sodium Azide
• Concentration: 80 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

RELA, NF-kappa-B p65delta3, NF-kappaB, NFKB3, p65, Transcription factor p65, NF kappaB, NFKB

Quick Database Links

Gene Symbol

RELA / NFKB p65