REG3A Antibody (N-term)

SKU: orb1928041

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: WB - 1:1000, FC - 1:10-50
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD43754
• Immunogen: This REG3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-39 amino acids from the N-terminal region of human REG3A. Antigen Region: 12-39 aa.
• Target: REG3A (HGNC:8601)
• Molecular Weight: 19395 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Regenerating islet-derived protein 3-alpha, REG-3-alpha, Hepatointestinal pancreatic protein, HIP/PAP, Human proislet peptide, Pancreatitis-associated protein 1, Regenerating islet-derived protein III-alpha, Reg III-alpha, Regenerating islet-derived protein 3-alpha 165 kDa form, Regenerating islet-derived protein 3-alpha 15 kDa form, REG3A, HIP, PAP, PAP1

REG3A Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of lysate from RPMI-8226 cell line, using REG3A Antibody (N-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35 ug per lane.

All lanes: Anti-REG3A Antibody (N-term) at 1:1000 dilution. Lane 1: Human pancreas tissue lysate. Lane 2: PANC-1 whole cell lysate. Lane 3: U-2OS whole cell lysate. Lane 4: A431 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 19 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

REG3A (HGNC:8601)

UniProt

Q06141

RefSeq (Protein):NP_620355.1, NP_002571.1, NP_620354.1