RECQL4 Antibody(C-term)

• Catalog Number: orb1932309
• Category: Antibodies
• Description: RECQL4 Antibody(C-term)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: O94761
• MW: 133067 Da
• Tested applications: IHC-P, WB
• Dilution range: WB - 1:2000
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: RECQ4
• Note: For research use only

Immunohistochemical analysis on paraffin-embedded Human tonsil tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

All lanes: Anti-RECQL4 Antibody (C-term) at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: A431 whole cell lysate. Lane 3: U-251 MG whole cell lysate. Lane 4: HepG2 whole cell lysate. Lane 5: Mouse testis tissue lysate. Lane 6: NIH/3T3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 133 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.