Recombinant VIM Antibody / Vimentin

• Catalog Number: orb2635162
• Category: Antibodies
• Description: When used in conjunction with anti-keratin staining, anti-vimentin staining is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma.
• Clonality: Recombinant
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A portion of amino acids 366-466 was used as the immunogen for the recombinant VIM antibody.
• UniProt ID: P08670
• Tested applications: IHC-P, WB
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml,Western blot: 1-2ug/ml
• Application notes: Optimal dilution of the recombinant VIM antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: rVIM/6914
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This recombinant VIM antibody is available for research use only.
• Note: For research use only

Western blot testing of human U-87 MG cell lysate using recombinant VIM antibody (clone rVIM/6914). Predicted molecular weight ~53 kDa.

IHC staining of FFPE human tonsil tissue with recombinant VIM antibody (clone rVIM/6914). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant VIM antibody (clone rVIM/6914) as confirmation of integrity and purity.