Recombinant Ubiquitin (Autophagy Marker) Antibody

• Catalog Number: orb607784
• Category: Antibodies
• Description: Rabbit monoclonal antibody to Ubiquitin (Autophagy Marker)
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: UBB/3143R
• Tested applications: IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant fragment of human Ubiquitin protein (around aa 1-119) (exact sequence is proprietary)
• Antibody Type: Recombinant Antibody
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Dilution range: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: 9kDa
• Target: CYP1A2
• Entrez: 7314
• UniProt ID: P62987, P62988, P62979
• Expression System: CHO cells
• Storage: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Buffer/Preservatives: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Alternative names: Epididymis secretory protein Li 50; Polyubiquitin
• Note: For research use only
• Application notes: Positive Control: HeLa or Jurkat cells. Alzheimer's Brain
• Expiration Date: 12 months from date of receipt.