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Description
Research Area
Cancer Biology
Images & Validation
−Item 1 of 2
| Tested Applications | IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Recombinant Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone No. | SMAD/6309R |
| Immunogen | Recombinant human full-length (aa1-552) SMAD4 protein |
| Target | SMAD4 |
| Source/Expression System | HEK293 cells |
| Molecular Weight | 61kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Deleted in Pancreatic Carcinoma 4 (DPC4); JIP; MAD homolog 4 (MADH4); Mothers against decapentaplegic homolog 4 (MADH4); Mothers against DPP homolog 4; MYHRS; SMAD4
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Formalin-fixed, paraffin-embedded human small intestine stained with SMAD4 / DPC4 Recombinant Rabbit Monoclonal Antibody (SMAD4/6309R). HIER: Tris/EDTA, pH9.0, 45 min. 2°C: HRP-polymer, 30 min. DAB, 5 min.
Immunohistochemical stain using SMAD4/6309R showing loss of SMAD4 expression in pancreatic ductal adenocarcinoma. Note adjacent benign ductal epithelium and background stroma as internal control showing positive / retained SMAD4 staining.
Quick Database Links
Gene Symbol
SMAD4
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
SMAD4 Antibody (orb1410509)
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