Recombinant Sarcomeric Alpha Actinin Antibody / Alpha Actinin 2 / ACTN2

• Catalog Number: orb2635696
• Category: Antibodies
• Description: Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In non-muscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for this gene.
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: Rabbit IgG, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A portion of amino acids 557-692 from the human protein was used as the immunogen for the recombinant Sarcomeric Alpha Actinin antibody.
• UniProt ID: P35609
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Application notes: Optimal dilution of the recombinant Sarcomeric Alpha Actinin antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: ACTN2/7041R
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This recombinant Sarcomeric Alpha Actinin antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human skeletal muscle with recombinant Sarcomeric Alpha Actinin antibody (clone ACTN2/7041R). Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant Sarcomeric Alpha Actinin antibody (clone ACTN2/7041R) as confirmation of integrity and purity.