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Catalog Number | orb2816785 |
---|---|
Category | Proteins |
Description | Recombinant Rat VEGF164 |
Tested applications | ELISA, WB |
Reactivity | Rat |
Tag | C-terminal His Tag |
Purity | Greater than 90% as determined by SDS-PAGE. |
MW | 23 kDa |
UniProt ID | P16612 |
Source | Mammalian Cells |
Expression Region | 27-190 |
Storage | The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 150 mM NaCl with 5% trehalose, pH 7.4. |
Alternative names | VEGF, VEGF-A, VEGF164 Read more... |
Note | For research use only |
Application notes | Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex. |
Expiration Date | 6 months from date of receipt. |
99.50% | |
19.2 kDa (predicted); 25 kDa (reducing condition, due to glycosylation) |
> 95% by SDS-PAGE. | |
Recombinant Rat VEGFA/VEGF164 Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Ala27-Arg190) of Rat VEGFA (Accession #NP_001274037.1) fused with an 6��His Tag at the C-terminus. |
ELISA, WB | |
Greater than 90% as determined by SDS-PAGE | |
23 kDa | |
Mammalian Cells |
ELISA, WB | |
Greater than 90% as determined by SDS-PAGE | |
21.1 kDa | |
E.Coli |