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Catalog Number | orb2648247 |
---|---|
Category | Proteins |
Description | Recombinant Rat VEGF164 |
Tested applications | ELISA, WB |
Reactivity | Rat |
Tag | N-terminal His Tag |
Purity | Greater than 90% as determined by SDS-PAGE |
MW | 21.1 kDa |
UniProt ID | P16612 |
Source | E.Coli |
Expression Region | 27-190 |
Storage | The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week under sterile condition. For extended storage, it is recommended to further dilute in working aliquots, store at -20°C to -80°C for 3 months. Avoid repeated freeze/thaw cycle. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 150 mM NaCl with 5% trehalose, pH 7.4. |
Alternative names | VEGF, VEGF-A, VEGF164 Read more... |
Note | For research use only |
Application notes | Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex. |
Expiration Date | 6 months from date of receipt. |
99.50% | |
19.2 kDa (predicted); 25 kDa (reducing condition, due to glycosylation) |
> 95% by SDS-PAGE. | |
Recombinant Rat VEGFA/VEGF164 Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Ala27-Arg190) of Rat VEGFA (Accession #NP_001274037.1) fused with an 6��His Tag at the C-terminus. |
ELISA, WB | |
Greater than 90% as determined by SDS-PAGE | |
23 kDa | |
Mammalian Cells |
ELISA, WB | |
Greater than 90% as determined by SDS-PAGE. | |
23 kDa | |
Mammalian Cells |