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Recombinant protein A
Description
Images & Validation
−Key Properties
−| Expression System | E.coli |
|---|---|
| Target | Immunoglobulin G-binding protein A |
| Tag | Tag free |
| Molecular Weight | 45-47 kDa |
| Protein Sequence | 37-469/516 |
| Purification | AC |
| Purity | >95% as determined by SDS-PAGE |
| Endotoxins | Not analyzed |
Storage & Handling
−| Storage | Stored at -70°C or -20°C. Avoid repeated freeze/thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Lyophilized from 0.22um filtered solution in PBS (pH7.4) with 5mM DTT. Normally 5% trehalose is added as protectant before Lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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The purity of the protein is greater than 90% as determined by reducing SDS-PAGE.
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Protocol Information
Recombinant protein A (orb86197)
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