Recombinant p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker) Antibody

SKU: orb750918

Description

Mouse monoclonal antibody against p63 conjugated to Biotin

Images & Validation

• Tested Applications: FC, IF, IHC
• Dilution range: Flow Cytometry (2-4ug/million cells); Immunofluorescence (2-4ug/ml);,Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Reactivity: Human
• Application Notes:
  Positive Control: HeLa cells. Human prostate or lung tissue (IHC)

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: rTP40/3690
• Immunogen: A synthetic peptide from the N-terminal of human p40 protein (Exact sequence is proprietary)
• Target: TP63
• Source/Expression System: HEK293 cells
• Molecular Weight: 40kDa
• Conjugation: Biotin

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Buffer/Preservatives: Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
• Concentration: Purified Ab conjugated to Biotin
• Disclaimer: For research use only

Alternative Names

Anti-deltaNp63 antibody, anti-TP63 antibody

Flow Cytometric Analysis of PFA-fixed HeLa cells using p40 Recombinant Mouse Monoclonal Antibody (rTP40/3690) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Formalin-fixed, paraffin-embedded human prostate stained with Biotinylated p40 Recombinant Mouse Monoclonal Antibody (rTP40/3690).

Quick Database Links

Gene Symbol

TP63

UniProt

Q9H3D4

NCBI Gene ID

8626