Recombinant p21WAF1 (Tumor Suppressor Protein) Antibody

• Catalog Number: orb751295
• Category: Antibodies
• Description: Rabbit monoclonal antibody to CDKN1A
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: CIP1/4377R
• Tested applications: IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Synthetic peptide corresponding to p21 residues within aa1-100 of p21 was used as an immunogen
• Antibody Type: Recombinant Antibody
• Concentration: 200 μg/ml
• Dilution range: Western Blot (2-4ug/ml); ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: 21kDa
• Target: CDKN1A
• Entrez: 1026
• UniProt ID: P38936
• Expression System: HEK293 cells
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Alternative names: Anti-Activating Fragment 1 antibody, anti-CAP20 an
• Note: For research use only
• Application notes: Positive Control: HeLa cells. Human skin, colon, or breast carcinoma.
• Expiration Date: 12 months from date of receipt.