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Description
Research Area
Immunology & Inflammation
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Mouse |
| Biological Activity | ELISA: Immobilized Human MICA-Fc(Cat#orb2994621)at 10μg/ml (100 μl/well)can bind Mouse NKG2D-Fc*(Cat#orb2993888)*Biotinylated by NHS-biotin prior to testing. Immobilized Anti-Human NKG2D mAb(Cat#orb2993153) at 2μg/ml (100 μl/well)can bind Mouse NKG2D-Fc(Cat#orb2993888).The ED50 of Mouse NKG2D-Fc(Cat#orb2993888) is 0.42ug/ml.. BLI: Loaded Mouse NKG2D-Fc(Cat#orb2993888) on Protein A Biosensor, can bind Human MICA-His(Cat#orb2995041) with an affinity constant of 0.42 uM as determined in BLI assay. (Regularly tested) |
| Tag | N-Fc |
| Expression Region | Phe94 - Val232 |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
| MW | Predicted: 42.2 KDa. Observed: 45-60 KDa, reducing conditions |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CD314; KLRK1;CD314 antigen;Killer cell lectin-like receptor subfamily K member 1; killer cell lectin-like receptor subfamily K; member 1; KLR; NK cell receptor D; NKG2-D; NKG2-D type II integral membrane protein; NKG2-D-activating NK receptor
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Quick Database Links
UniProt
UniProt Details
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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