Recombinant Mouse NKG2D Protein

SKU: orb2993888

Description

Recombinant Mouse NKG2-D Type II Integral Membrane Protein is produced by our Mammalian expression system and the target gene encoding Phe94 - Val232 is expressed with a human IgG1 Fc tag at the N-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Mouse
• Biological Activity: ELISA: Immobilized Human MICA-Fc(Cat#orb2994621)at 10μg/ml (100 μl/well)can bind Mouse NKG2D-Fc*(Cat#orb2993888)*Biotinylated by NHS-biotin prior to testing. Immobilized Anti-Human NKG2D mAb(Cat#orb2993153) at 2μg/ml (100 μl/well)can bind Mouse NKG2D-Fc(Cat#orb2993888).The ED50 of Mouse NKG2D-Fc(Cat#orb2993888) is 0.42ug/ml.. BLI: Loaded Mouse NKG2D-Fc(Cat#orb2993888) on Protein A Biosensor, can bind Human MICA-His(Cat#orb2995041) with an affinity constant of 0.42 uM as determined in BLI assay. (Regularly tested)
• Tag: N-Fc
• Molecular Weight: Predicted: 42.2 KDa. Observed: 45-60 KDa, reducing conditions
• Expression Region: Phe94 - Val232
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD314; KLRK1;CD314 antigen;Killer cell lectin-like receptor subfamily K member 1; killer cell lectin-like receptor subfamily K; member 1; KLR; NK cell receptor D; NKG2-D; NKG2-D type II integral membrane protein; NKG2-D-activating NK receptor

Quick Database Links

UniProt

O54709