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Catalog Number | orb689424 |
---|---|
Category | Proteins |
Description | Recombinant human NKG2D protein with N-terminal mouse Fc |
Target | NKG2D |
Tag | N-Mouse Fc tag |
Reactivity | Human |
Form/Appearance | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
Buffer/Preservatives | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. |
Purity | The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
Purification | Purified from cell culture supernatant by affinity chromatography |
UniProt ID | P26718 |
MW | The protein has a predicted molecular mass of 42.8 kDa after removal of the signal peptide.The apparent molecular mass of mFc-NKG2D is approximately 50-65 kDa due to glycosylation. |
Source | Mammalian |
Storage | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Alternative names | NKG2D;CD314;KLRK1;NK cell receptor D |
Note | For research use only |
Human 2B4, hFc Tag on SDS-PAGE under reducing condition.
ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human CD48, mFc-His tagged protein orb689377 can bind Human 2B4, hFc tagged protein (orb689461) in a linear range of 62.5-4000 ng/ml.
98.00% | |
36.8 kDa (predicted). Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result. |
98.00% | |
41.83 kDa (predicted); 46.51 kDa (reducing conditions) |