Recombinant Human VEGFR2 Protein

SKU: orb2994455

Description

Recombinant Human Vascular Endothelial Growth Factor Receptor 2 is produced by our Mammalian expression system and the target gene encoding Ala20-Glu764 is expressed with a human IgG1 Fc tag at the C-terminus.

Research Area

Cardiovascular Research; Cell Biology

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Human VEGF121-His (Cat#orb2994931) at 10μg/ml (100 μl/well) can bind Human VEGFR2-Fc (Cat#orb2994455). The ED50 of Recombinant Human VEGFR2-Fc (Cat#orb2994455) is 1.92 ug/ml. Immobilized Human VEGF 121(Cat#orb2994925) at 2μg/ml (100 μl/well)can bind Human VEGFR2-Fc(Cat#orb2994455) .The ED50 of Human VEGFR2-Fc(Cat#orb2994455) is 13.04 ng/ml.
• Tag: C-Fc
• Expression Region: Ala20-Glu764
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated
• MW: Predicted: 110.4 KDa. Observed: 150-160 KDa, reducing conditions
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of 20 mM Tris-HCl, 15% Maltose, 0.05% Tween 80, pH 9.0.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Vascular endothelial growth factor receptor 2; KDR; VEGFR-2; Fetal liver kinase 1; FLK-1; Kinase insert domain receptor; Protein-tyrosine kinase receptor flk-1

Quick Database Links

UniProt

P35968