Biotinylated Human VEGFR2

SKU: orb2993796

Description

Biotinylated Recombinant Human Vascular Endothelial Growth Factor Receptor 2 is produced by our Mammalian expression system and the target gene encoding Ala20-Glu764 is expressed with a 6His, Avi tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Biotinylated Human VEGFR2-His-Avi(Cat#orb2993796) at 1μg/ml (100 μl/well)can bind Anti-Human VEGFR2 mAb(Cat#orb2993814).The ED50 of Anti-Human VEGFR2 mAb(Cat#orb2993814) is 44.08 ng/ml.. BLI: Loaded Anti-Human VEGFR2 mAb-Fc(Cat#orb2993814) on Protein A Biosensor, can bind Biotinylated Human VEGFR2-6His-Avi(Cat#orb2993796) with an affinity constant of 1.1 pM as determined in BLI assay.
• Tag: C-6His-Avi
• Molecular Weight: Predicted: 86.1 KDa. Observed: 120-150 KDa, reducing conditions
• Expression Region: Ala20-Glu764
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Biotin

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Disclaimer: For research use only

Alternative Names

Vascular endothelial growth factor receptor 2; KDR; VEGFR-2; Fetal liver kinase 1; FLK-1; Kinase insert domain receptor; Protein-tyrosine kinase receptor flk-1

Quick Database Links

UniProt

P35968