Recombinant Human Vascular endothelial growth factor A, long form (VEGFA) (Active)

SKU: orb2657906

Description

This Recombinant Human Vascular endothelial growth factor A, long form (VEGFA) (Active) spans the amino acid sequence from region 27-191aa. Purity: Greater than 95% as determined by SDS-PAGE.

Research Area

Cardiovascular Research; Stem Cell & Developmental Biology; Cell Biology

Images & Validation

• Application Notes:
  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Key Properties

• Source: Mammalian cell
• Biological Origin: Homo sapiens (Human)
• Biological Activity: Measured in a cell proliferation assay using HUVEC human umbilic vein endothelial cells. The ED50 for this effect is 0.2-10 ng/mL.
• Tag: C-terminal 6xHis-tagged
• Molecular Weight: 20 kDa
• Expression Region: 27-191aa
• Protein Length: Full Length of Mature Protein of Isoform VEGF165
• Protein Sequence: APMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR
• Purity: Greater than 95% as determined by SDS-PAGE.
• Endotoxins: ≤10 EU/mg by the LAL method

Storage & Handling

• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
• Form/Appearance: Lyophilized powder
• Buffer/Preservatives: Lyophilized from a 0.2 μm filtered solution containing PBS, 5% mannitoland 0.01% Tween 80, pH 7.4
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Vascular Endothelial Growth Factor Isoform 165; VEGF165

Quick Database Links

UniProt

P15692