Recombinant Human TPBG Protein

SKU: orb2994942

Description

Recombinant Human Trophoblast Glycoprotein is produced by our Mammalian expression system and the target gene encoding Ser32-Ser355 is expressed with a 6His tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Recombinant TPBG antibody (naptumomab) (Cat#orb2992640) at 2 μg/ml (100 μl/well) can bind Recombinant Human TPBG (C-6His) (Cat#orb2994942). The ED50 of Recombinant Human TPBG (C-6His) (Cat#orb2994942) is 14.45 ng/ml. (Regularly tested). BLI: Loaded Human TPBG Antibody (5G9) (Human IgG1)(Cat#orb2992640) on Protein-A Biosensor, can bind Human TPBG (C-6His) (Cat#orb2994942) with an affinity constant of 8.19 nM as determined in BLI assay. (Regularly tested)
• Tag: C-6His
• Expression Region: Ser32-Ser355
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated
• MW: Predicted: 36.1 KDa. Observed: 55-80 KDa, reducing conditions
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of PBS, pH 7.4 .
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

5T4 Protein; 5T4AG Protein; M6P1 Protein; WAIF1 Protein; TPBG

Quick Database Links

UniProt

Q13641