Biotinylated Human 5T4

SKU: orb2993903

Description

Biotinylated Recombinant Human Trophoblast Glycoprotein is produced by our Mammalian expression system and the target gene encoding Ser32-Ser355 is expressed with a Avi, 6His tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Human TPBG Antibody (5G9) (Human IgG1) (Cat#orb2992640) at 2 μg/ml (100 μl/well) can bind Biotinylated Human TPBG-Avi-His(Cat#orb2993903). The ED50 of Human Biotinylated TPBG-Avi-His (Cat#orb2993903) is 0.21 ng/ml. (QC verified).. BLI: LoadedHuman TPBG Antibody (5G9) (Human IgG1)(Cat#orb2992640) on Protein-A Biosensor, can bind Biotinylated Human 5T4 (C-Avi-6His) (Cat#orb2993903) with an affinity constant of 9.29 nM as determined in BLI assay. (Regularly tested)
• Tag: C-Avi-6His
• Molecular Weight: Predicted: 38 KDa. Observed: 50-90 KDa, reducing conditions
• Expression Region: Ser32-Ser355
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Biotin

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TPBG; 5T4; 5T4AG; M6P1; WAIF1

Quick Database Links

UniProt

Q13641