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Description
Research Area
Immunology & Inflammation
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Recombinant Human TNF RII-mFc(Cat#orb2994890) at 1μg/ml (100 μl/well) can bind Recombinant Human TNF alpha-His(Cat#orb3002254). The ED50 of Recombinant Human TNF alpha-His(Cat#orb3002254) is 1.97 ng/ml. (Regularly tested). BLI: Loaded Human TNF alpha-His(Cat#orb3002254) on HIS1K Biosensor, can bind Human TNF RII-mFc(Cat#orb2994890) with an affinity constant of 1.83 nM as determined in BLI assay. (Regularly tested) |
| Tag | C-mFc |
| Expression Region | Pro24-Thr206 |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
| MW | Predicted: 46.44 KDa. Observed: 60 KDa, reducing conditions |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Tumor necrosis factor receptor superfamily member 1B; TNFRSF1B; Tumor necrosis factor receptor 2; TNF-R2; TNF-RII; Tumor necrosis factor receptor type II; p75; p80 TNF-alpha receptor; CD120b
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