Human TNF RII

SKU: orb2994311

Description

Recombinant Human Tumor Necrosis Factor Receptor II is produced by our Mammalian expression system and the target gene encoding Leu23-Asp257 is expressed with a human IgG1 Fc tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Recombinant Human TNF alpha (Cat#orb3002485) at 5μg/ml (100 μl/well) can bind Recombinant Human TNF RII (C-Fc) (Cat#orb2994311). The ED50 of Recombinant Human TNF RII (C-Fc) (Cat#orb2994311) is 15.35 ng/ml. (Regularly tested). BLI: Loaded Recombinant Human TNF RII (C-Fc) (Cat#orb2994311) on Pro-A Biosensor, can bind Recombinant Human TNF alpha (Cat#orb3002485) with an affinity constant of 25.2 pM as determined in BLI assay. (Regularly tested). Cell Based Assay: Measured by its ability to induce NF-κB reporter activity in HEK293 human embryonic kidney cells. Recombinant Human TNF RII (Cat# orb2994311) inhibits a constant dose of 0.5ng/mL of Recombinant TNF alpha(Cat#orb3002485). The IC50 for this effect is 2.5 ng/mL. (Regularly tested)
• Tag: C-Fc
• Molecular Weight: Predicted: 51.9 KDa. Observed: 60-90 KDa, reducing conditions
• Expression Region: Leu23-Asp257
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Disclaimer: For research use only

Alternative Names

Tumor necrosis factor receptor superfamily member 1B; TNFRSF1B; Tumor necrosis factor receptor 2; TNF-R2; TNF-RII; Tumor necrosis factor receptor type II; p75; p80 TNF-alpha receptor; CD120b

Quick Database Links

UniProt

P20333