Human TF

SKU: orb2992804

Description

Recombinant Human Tissue factor is produced by our Mammalian expression system and the target gene encoding Ser33-Glu251 is expressed with a human IgG1 Fc tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Anti-Human TF Antibody (TIS_bio, Research Grade)(Cat#orb2992713) at 1μg/ml (100 μl/well) can bind Recombinant Human TF (C-Fc)(Cat#orb2992804)*. *: Biotinylated by NHS-biotin prior to testing.The EC50 of Recombinant Human TF (C-Fc)(Cat#orb2992804) is 2.71 ng/ml.(Regularly tested).
• Tag: C-Fc
• Molecular Weight: Predicted: 51.6 KDa. Observed: 66-77 KDa, reducing conditions
• Expression Region: Ser33-Glu251
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
• Endotoxins: < 1 EU/µg as determined by LAL test.

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Tissue Factor; TF; Coagulation Factor III; Thromboplastin; CD142; F3

Quick Database Links

UniProt

P13726