Human TF

SKU: orb2995049

Description

Recombinant Human Coagulation Factor III/Tissue Factor is produced by our Mammalian expression system and the target gene encoding Gly34-Glu251 is expressed with a 6His tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Recombinant Human TF (C-6His)(Cat#orb2995049) at 1μg/ml (100 μl/well) can bind Anti-Human TF Antibody (TIS_bio, Research Grade)(Cat#orb2992713).The EC50 of Anti-Human TF Antibody (TIS_bio, Research Grade)(Cat#orb2992713) is ng/ml.(Regularly tested).. BLI: Loaded Anti-TF-mAb on Pro-A Biosensor, can bind Recombinant Human TF (C-6His) (Cat#orb2995049) with an affinity constant of 9.69 nM as determined in BLI assay. (Regularly tested)
• Tag: C-6His
• Molecular Weight: Predicted: 25.76 KDa. Observed: 35-55 KDa, reducing conditions
• Expression Region: Gly34-Glu251
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Tissue Factor; TF; Coagulation Factor III; Thromboplastin; CD142; F3

Quick Database Links

UniProt

P13726