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Catalog Number | orb1516850 |
---|---|
Category | Proteins |
Description | Recombinant human PEDF protein, His |
Target | Pigment epithelium-derived factor |
Tag | His |
Form/Appearance | Lyophilized or Liquid |
Concentration | >0.5 mg/ml |
Buffer/Preservatives | 20mM Tris-HCl (pH8.0) with 8M Urea. |
Purity | > 95% as determined by SDS-PAGE |
Protein Sequence | 20-418/418 |
MW | 55 kDa |
Application notes | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Expression System | E.coli |
Endotoxins | Not analyzed |
Biological Activity | Not tested |
Storage | Stored at -70°C or -20°C. Avoid repeated freeze/thaw cycles. |
Alternative names | pigment epithelium-derived factor; Serpin-F1; Stro Read more... |
Note | For research use only |
The purity of the protein is greater than 90% as determined by reducing SDS-PAGE.
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 45.2 kDa after removal of the signal peptide. The apparent molecular mass of PEDF-His is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
Greater than 90% as determined by SDS-PAGE. | |
Escherichia Coli |
98.00% | |
45.8 kDa (predicted); 45.8 kDa (reducing conditions) |