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Catalog Number | orb427611 |
---|---|
Category | Proteins |
Description | Recombinant of human PEDF (His) protein |
Form/Appearance | Sterile Filtered colorless solution. |
Buffer/Preservatives | The PEDF solution contains 20mM Tris-HCl buffer(pH 8.0), 0.1M NaCl , and 20% glycerol. |
Purity | Greater than 90% as determined by SDS-PAGE. |
Protein Sequence | MGSSHHHHHH SSGLVPRGSH MQNPASPPEE GSPDPDSTGA LVEEEDPFFK VPVNKLAAAV SNFGYDLYRV RSSMSPTTNV LLSPLSVATA LSALSLGAEQ RTESIIHRAL YYDLISSPDI HGTYKELLDT VTAPQKNLKS ASRIVFEKKL RIKSSFVAPL EKSYGTRPRV LTGNPRLDLQ EINNWVQAQM KGKLARSTKE IPDEISILLL GVAHFKGQWV TKFDSRKTSL EDFYLDEERT VRVPMMSDPK AVLRYGLDSD LSCKIAQLPL TGSMSIIFFL PLKVTQNLTL IEESLTSEFI HDIDRELKTV QAVLTVPKLK LSYEGEVTKS LQEMKLQSLF DSPDFSKITG KPIKLTQVEH RAGFEWNEDG AGTTPSPGLQ PAHLTFPLDY HLNQPFIFVL RDTDTGALLF IGKILDPRGP |
Application notes | Cytokines And Growth Factors |
Source | Escherichia Coli |
Storage | Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles |
Alternative names | Pigment epithelium-derived factor, PEDF, Serpin-F1 Read more... |
Note | For research use only |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 45.2 kDa after removal of the signal peptide. The apparent molecular mass of PEDF-His is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
> 95% as determined by SDS-PAGE | |
55 kDa |
98.00% | |
45.8 kDa (predicted); 45.8 kDa (reducing conditions) |
> 98%, determined by SDS-PAGE | |
This protein contains the human SERPINF1 (NP_0026063) (Met 1-Pro 418) was fused with a polyhistidine tag at the C-terminus and expressed from HEK293 Cells. |