Recombinant Human PD-1 Protein

SKU: orb2993958

Description

Recombinant Human Programmed Cell Death Protein 1 is produced by our Mammalian expression system and the target gene encoding Pro21-Gln167 is expressed with a 6His tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Human PD-1-His (Cat#orb2993958) at 2μg/ml (100 μl/well) can bind Anti-PD1 mAb (Cat#GMP-A085). The ED50 of Anti-PD1 mAb (Cat#GMP-A085) is 9.74 ng/ml. (Regularly tested) Immobilized Recombinant Human PD-1-His(Cat#orb2993958) at 5μg/ml (100 μl/well)can bind Human PD-L1-Fc-Avi(Cat#orb2994143). The ED50 of Human PD-L1-Fc-Avi(Cat#orb2994143) is 0.185 ug/ml.. BLI: Loaded Anti-Human PD-1 mAb-Fc(Cat#GMP-A085) on Protein A Biosensor, can bind Human PD-1-His(Cat#orb2993958) with an affinity constant of 4.08 nM as determined in BLI assay.
• Tag: C-6His
• Molecular Weight: Predicted: 17 KDa. Observed: 30-42 KDa, reducing conditions
• Expression Region: Pro21-Gln167
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of 20mM Tris-HCl, 10% Trehalose, 100mM NaCl, 0.05% Tween 80, pH 7.5.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Programmed cell death protein 1; hPD-1; PDCD1; CD279

Quick Database Links

UniProt

Q15116