Recombinant Human PD-1 Protein

SKU: orb2994332

Description

Recombinant Human Programmed Cell Death Protein 1 mutant(C93S) is produced by our Mammalian expression system and the target gene encoding Leu25-Gln167(Cys93Ser) is expressed with a 6His tag at the C-terminus.

Research Area

Cancer Biology

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: BLI: Loaded Anti-Human PD-1 mAb-Fc(Cat#GMP-A085) on Protein A Biosensor, can bind Human PD-1-His(Cat#orb2994332) with an affinity constant of 4.97 nM as determined in BLI assay.
• Tag: C-6His
• Expression Region: Leu25-Gln167(Cys93Ser)
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated
• MW: Predicted: 16.8 KDa. Observed: 23-38 KDa, reducing conditions
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Programmed cell death protein 1; PDCD1; PD-1; hPD-1; CD279

Quick Database Links

UniProt

Q15116