Recombinant Human OX40 Protein

SKU: orb2994421

Description

Recombinant Human OX40L Receptor is produced by our Mammalian expression system and the target gene encoding Leu29-Ala216 is expressed with a human IgG1 Fc tag at the N-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Mouse OX40L-His(Cat#orb2994337) at 10μg/ml (100 μl/well) can bind Human OX40-Fc(Cat#orb2994421).The ED50 of Human OX40-Fc(Cat#orb2994421)is 0.10 ug/ml.. BLI: Loaded Biotinylated Mouse OX40L-His(Cat#orb2994337) on AR2G Biosensor, can bind Human OX40-Fc(Cat#orb2994421) with an affinity constant of 907 nM as determined in BLI assay.
• Tag: N-Fc
• Molecular Weight: Predicted: 46.3 KDa. Observed: 40-60 KDa, reducing conditions
• Expression Region: Leu29-Ala216
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Tumor necrosis factor receptor superfamily member 4;TNFRSF4;OX40;CD134;Txgp1

Quick Database Links

UniProt

P43489